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biorad aurum kits  (Bio-Rad)


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    Structured Review

    Bio-Rad biorad aurum kits
    Biorad Aurum Kits, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 2836 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/aurum+total+rna+mini+kit+biorad/Aurum+Total+RNA+Mini+Kit/bio_rxiv__64898__2026__03__27__714373-426-5-5
    Average 96 stars, based on 2836 article reviews
    biorad aurum kits - by Bioz Stars, 2026-10
    96/100 stars

    Images

    Related Articles

    cDNA Synthesis:

    Article Title: Rickettsial pathogen inhibits tick cell death through tryptophan metabolite mediated activation of p38 MAP kinase.
    Article Snippet: N, N-diethyl-p-phenylenediamine (DE-PPD) Alfa Aesar Cat # A17832 Horseradish peroxidase (POD) ThermoScientific, USA Cat #J60026.MC Kynurenic acid (KA) Sigma, USA Cat #K3375-1G Hydrogen peroxide ThermoScientific, USA Cat # BP2633-500 Protein Plus ladder BioRad, USA Cat # 1610373 .. Aurum Total RNA Mini kit BioRad, USA Cat # 732-6820 DNeasy Blood & Tissue Kit Qiagen Cat # 69506 iScript cDNA synthesis kit BioRad, USA Cat # 1708891 Bradford (BCA) protein assay kit Pierce/ThermoScientific, USA Cat # 23227 LIVE/DEAD Cell Imaging kit ThermoFisher Scientific Cat #R37601 Experimental models: Cell lines Tick cell line ISE6 ATCC Cat # CRL-11974 .. Ticks- Ixodes scapularis BEI Resources, NIAID, NIH NR-44115, NR-42510, NR-44116

    Article Title: Pyrazole[3,4-e][1,4]thiazepin-7-one derivatives as a novel class of Farnesoid X Receptor (FXR) agonists
    Article Snippet: A virtual screening procedure was applied to the discovery of structurally diverse non-steroidal Farnesoid X Receptor (FXR) agonists.. From 117 compounds selected by virtual screening, a total of 47 compounds were found to be FXR agonists, with 34 of them showing activity below a concentration of 20 lM.. 1H-Pyrazole[3,4-e][1,4]thiazepin-7-one-based hit compound 7 was chosen for hit-to-lead optimization.


    Bicinchoninic Acid Protein Assay:

    Article Title: Rickettsial pathogen inhibits tick cell death through tryptophan metabolite mediated activation of p38 MAP kinase.
    Article Snippet: N, N-diethyl-p-phenylenediamine (DE-PPD) Alfa Aesar Cat # A17832 Horseradish peroxidase (POD) ThermoScientific, USA Cat #J60026.MC Kynurenic acid (KA) Sigma, USA Cat #K3375-1G Hydrogen peroxide ThermoScientific, USA Cat # BP2633-500 Protein Plus ladder BioRad, USA Cat # 1610373 .. Aurum Total RNA Mini kit BioRad, USA Cat # 732-6820 DNeasy Blood & Tissue Kit Qiagen Cat # 69506 iScript cDNA synthesis kit BioRad, USA Cat # 1708891 Bradford (BCA) protein assay kit Pierce/ThermoScientific, USA Cat # 23227 LIVE/DEAD Cell Imaging kit ThermoFisher Scientific Cat #R37601 Experimental models: Cell lines Tick cell line ISE6 ATCC Cat # CRL-11974 .. Ticks- Ixodes scapularis BEI Resources, NIAID, NIH NR-44115, NR-42510, NR-44116

    Article Title: Sleep fragmentation induces heart failure in a hypertrophic cardiomyopathy mouse model by altering redox metabolism.
    Article Snippet: Antibodies CLOCK (D45B10) Rabbit mAb Cell Signaling Technology Cat#5157; RRID:AB_10695411 BMAL1 (D2L7G) Rabbit mAb Cell Signaling Technology Cat#14020; RRID:AB_2728705 Cytochrome c (136F3) Rabbit mAb Cell Signaling Technology Cat#4280; RRID:AB_10695410 VDAC (D73D12) Rabbit mAb Cell Signaling Technology Cat#4661; RRID:AB_10557420 Cleaved Caspase-9 (Asp330) (E5Z7N) Rabbit mAb Cell Signaling Technology Cat#52873; RRID:AB_2799423 Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb Cell Signaling Technology Cat#9664; RRID:AB_2070042 Anti-NRF2 (D1Z9C) XP Rabbit monoclonal Antibody Cell Signaling Technology Cat#12721; RRID:AB_2715528 Catalase (D4P7B)XP Rabbit mAb Cell Signaling Technology Cat#12980; RRID:AB_2798079 GST-Tag (26H1) Mouse mAb Cell Signaling Technology Cat#2624; RRID:AB_2189875 GAPDH (D16H11) XP Rabbit mAb Cell Signaling Technology Cat#5174; RRID:AB_10622025 Anti-PER1 antibody - N-terminal Abcam Cat#ab136451; RRID:AB_3075441 Anti-mCherry antibody Abcam Cat# ab167453; RRID:AB_2571870 Anti-Creatine kinase MT antibody Abcam Cat#ab198257, RRID:AB_3075442 TrxR2 Recombinant Rabbit Monoclonal Antibody (ARC1339) ThermoFisher Scientific Cat#MA5-35730, RRID:AB_2849630 ANTI-MYBPC3 (N-TERM) antibody Sigma-Aldrich Cat#SAB1303629-400UL; RRID:AB_3075444 Chemicals, peptides, and recombinant proteins Hydrogen peroxide solution Sigma Cat#88597 Phosphate Buffer Solution Sigma Cat#P3619 2-Thiobarbituric acid Sigma Cat#T5500 Glutaraldehyde solution Sigma Cat#G5882 .. Glutathione Assay Kit Cayman chemical Cat#703002 Bio-Rad Protein Assay Kit II Biorad Cat#5000002 Glucose-6-Phosphate Dehydrogenase Assay Kit (Colorimetric) Abcam Cat#ab102529 Aurum Total RNA Mini Kit Biorad Cat7326820 iScript Reverse Transcription Supermix for RT-qPCR Biorad Cat#1708841 Pierce BCA Protein Assay Kits ThermoFisher Scientific Cat# 23225 Experimental models: Organisms/strains Humanized MYBPC3 mice on C57BL/6 background This paper N/A Oligonucleotides See Table S1 for primers sequences This paper N/A Software and algorithms GraphPad Software San Diego, CA https://www.graphpad.com/ ImageJ National Institutes of Health (NIH) https://imagej.net/ij/ ..

    Imaging:

    Article Title: Rickettsial pathogen inhibits tick cell death through tryptophan metabolite mediated activation of p38 MAP kinase.
    Article Snippet: N, N-diethyl-p-phenylenediamine (DE-PPD) Alfa Aesar Cat # A17832 Horseradish peroxidase (POD) ThermoScientific, USA Cat #J60026.MC Kynurenic acid (KA) Sigma, USA Cat #K3375-1G Hydrogen peroxide ThermoScientific, USA Cat # BP2633-500 Protein Plus ladder BioRad, USA Cat # 1610373 .. Aurum Total RNA Mini kit BioRad, USA Cat # 732-6820 DNeasy Blood & Tissue Kit Qiagen Cat # 69506 iScript cDNA synthesis kit BioRad, USA Cat # 1708891 Bradford (BCA) protein assay kit Pierce/ThermoScientific, USA Cat # 23227 LIVE/DEAD Cell Imaging kit ThermoFisher Scientific Cat #R37601 Experimental models: Cell lines Tick cell line ISE6 ATCC Cat # CRL-11974 .. Ticks- Ixodes scapularis BEI Resources, NIAID, NIH NR-44115, NR-42510, NR-44116

    other:

    Article Title: Extending SAR of bile acids as FXR ligands: discovery of 23-N-(carbocinnamyloxy)-3α,7α-dihydroxy-6α-ethyl-24-nor-5β-cholan-23-amine.
    Article Snippet: Within our efforts in the discovery of novel potent and selective ligands for the FXR receptor, 23-N-(carbocinnamyloxy)-3a,7a-dihydroxy-6a-ethyl-24-nor-5b-cholan-23-amine was synthesized and evaluated for its ability to activate and modulate the biological response of the receptor.. Alphascreen and RT-PCR revealed that the 6a-ethyl-24-norcholanyl-23-amine derivate behaves as full FXR agonist endowed with high binding affinity and efficacy, representing a promising lead candidate for further optimization.. In addition, docking studies provide new insights into the molecular basis governing the partial and full agonist activity at FXR.

    Glutathione Assay:

    Article Title: Sleep fragmentation induces heart failure in a hypertrophic cardiomyopathy mouse model by altering redox metabolism.
    Article Snippet: Antibodies CLOCK (D45B10) Rabbit mAb Cell Signaling Technology Cat#5157; RRID:AB_10695411 BMAL1 (D2L7G) Rabbit mAb Cell Signaling Technology Cat#14020; RRID:AB_2728705 Cytochrome c (136F3) Rabbit mAb Cell Signaling Technology Cat#4280; RRID:AB_10695410 VDAC (D73D12) Rabbit mAb Cell Signaling Technology Cat#4661; RRID:AB_10557420 Cleaved Caspase-9 (Asp330) (E5Z7N) Rabbit mAb Cell Signaling Technology Cat#52873; RRID:AB_2799423 Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb Cell Signaling Technology Cat#9664; RRID:AB_2070042 Anti-NRF2 (D1Z9C) XP Rabbit monoclonal Antibody Cell Signaling Technology Cat#12721; RRID:AB_2715528 Catalase (D4P7B)XP Rabbit mAb Cell Signaling Technology Cat#12980; RRID:AB_2798079 GST-Tag (26H1) Mouse mAb Cell Signaling Technology Cat#2624; RRID:AB_2189875 GAPDH (D16H11) XP Rabbit mAb Cell Signaling Technology Cat#5174; RRID:AB_10622025 Anti-PER1 antibody - N-terminal Abcam Cat#ab136451; RRID:AB_3075441 Anti-mCherry antibody Abcam Cat# ab167453; RRID:AB_2571870 Anti-Creatine kinase MT antibody Abcam Cat#ab198257, RRID:AB_3075442 TrxR2 Recombinant Rabbit Monoclonal Antibody (ARC1339) ThermoFisher Scientific Cat#MA5-35730, RRID:AB_2849630 ANTI-MYBPC3 (N-TERM) antibody Sigma-Aldrich Cat#SAB1303629-400UL; RRID:AB_3075444 Chemicals, peptides, and recombinant proteins Hydrogen peroxide solution Sigma Cat#88597 Phosphate Buffer Solution Sigma Cat#P3619 2-Thiobarbituric acid Sigma Cat#T5500 Glutaraldehyde solution Sigma Cat#G5882 .. Glutathione Assay Kit Cayman chemical Cat#703002 Bio-Rad Protein Assay Kit II Biorad Cat#5000002 Glucose-6-Phosphate Dehydrogenase Assay Kit (Colorimetric) Abcam Cat#ab102529 Aurum Total RNA Mini Kit Biorad Cat7326820 iScript Reverse Transcription Supermix for RT-qPCR Biorad Cat#1708841 Pierce BCA Protein Assay Kits ThermoFisher Scientific Cat# 23225 Experimental models: Organisms/strains Humanized MYBPC3 mice on C57BL/6 background This paper N/A Oligonucleotides See Table S1 for primers sequences This paper N/A Software and algorithms GraphPad Software San Diego, CA https://www.graphpad.com/ ImageJ National Institutes of Health (NIH) https://imagej.net/ij/ ..

    Dehydrogenase Assay:

    Article Title: Sleep fragmentation induces heart failure in a hypertrophic cardiomyopathy mouse model by altering redox metabolism.
    Article Snippet: Antibodies CLOCK (D45B10) Rabbit mAb Cell Signaling Technology Cat#5157; RRID:AB_10695411 BMAL1 (D2L7G) Rabbit mAb Cell Signaling Technology Cat#14020; RRID:AB_2728705 Cytochrome c (136F3) Rabbit mAb Cell Signaling Technology Cat#4280; RRID:AB_10695410 VDAC (D73D12) Rabbit mAb Cell Signaling Technology Cat#4661; RRID:AB_10557420 Cleaved Caspase-9 (Asp330) (E5Z7N) Rabbit mAb Cell Signaling Technology Cat#52873; RRID:AB_2799423 Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb Cell Signaling Technology Cat#9664; RRID:AB_2070042 Anti-NRF2 (D1Z9C) XP Rabbit monoclonal Antibody Cell Signaling Technology Cat#12721; RRID:AB_2715528 Catalase (D4P7B)XP Rabbit mAb Cell Signaling Technology Cat#12980; RRID:AB_2798079 GST-Tag (26H1) Mouse mAb Cell Signaling Technology Cat#2624; RRID:AB_2189875 GAPDH (D16H11) XP Rabbit mAb Cell Signaling Technology Cat#5174; RRID:AB_10622025 Anti-PER1 antibody - N-terminal Abcam Cat#ab136451; RRID:AB_3075441 Anti-mCherry antibody Abcam Cat# ab167453; RRID:AB_2571870 Anti-Creatine kinase MT antibody Abcam Cat#ab198257, RRID:AB_3075442 TrxR2 Recombinant Rabbit Monoclonal Antibody (ARC1339) ThermoFisher Scientific Cat#MA5-35730, RRID:AB_2849630 ANTI-MYBPC3 (N-TERM) antibody Sigma-Aldrich Cat#SAB1303629-400UL; RRID:AB_3075444 Chemicals, peptides, and recombinant proteins Hydrogen peroxide solution Sigma Cat#88597 Phosphate Buffer Solution Sigma Cat#P3619 2-Thiobarbituric acid Sigma Cat#T5500 Glutaraldehyde solution Sigma Cat#G5882 .. Glutathione Assay Kit Cayman chemical Cat#703002 Bio-Rad Protein Assay Kit II Biorad Cat#5000002 Glucose-6-Phosphate Dehydrogenase Assay Kit (Colorimetric) Abcam Cat#ab102529 Aurum Total RNA Mini Kit Biorad Cat7326820 iScript Reverse Transcription Supermix for RT-qPCR Biorad Cat#1708841 Pierce BCA Protein Assay Kits ThermoFisher Scientific Cat# 23225 Experimental models: Organisms/strains Humanized MYBPC3 mice on C57BL/6 background This paper N/A Oligonucleotides See Table S1 for primers sequences This paper N/A Software and algorithms GraphPad Software San Diego, CA https://www.graphpad.com/ ImageJ National Institutes of Health (NIH) https://imagej.net/ij/ ..

    Reverse Transcription:

    Article Title: Sleep fragmentation induces heart failure in a hypertrophic cardiomyopathy mouse model by altering redox metabolism.
    Article Snippet: Antibodies CLOCK (D45B10) Rabbit mAb Cell Signaling Technology Cat#5157; RRID:AB_10695411 BMAL1 (D2L7G) Rabbit mAb Cell Signaling Technology Cat#14020; RRID:AB_2728705 Cytochrome c (136F3) Rabbit mAb Cell Signaling Technology Cat#4280; RRID:AB_10695410 VDAC (D73D12) Rabbit mAb Cell Signaling Technology Cat#4661; RRID:AB_10557420 Cleaved Caspase-9 (Asp330) (E5Z7N) Rabbit mAb Cell Signaling Technology Cat#52873; RRID:AB_2799423 Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb Cell Signaling Technology Cat#9664; RRID:AB_2070042 Anti-NRF2 (D1Z9C) XP Rabbit monoclonal Antibody Cell Signaling Technology Cat#12721; RRID:AB_2715528 Catalase (D4P7B)XP Rabbit mAb Cell Signaling Technology Cat#12980; RRID:AB_2798079 GST-Tag (26H1) Mouse mAb Cell Signaling Technology Cat#2624; RRID:AB_2189875 GAPDH (D16H11) XP Rabbit mAb Cell Signaling Technology Cat#5174; RRID:AB_10622025 Anti-PER1 antibody - N-terminal Abcam Cat#ab136451; RRID:AB_3075441 Anti-mCherry antibody Abcam Cat# ab167453; RRID:AB_2571870 Anti-Creatine kinase MT antibody Abcam Cat#ab198257, RRID:AB_3075442 TrxR2 Recombinant Rabbit Monoclonal Antibody (ARC1339) ThermoFisher Scientific Cat#MA5-35730, RRID:AB_2849630 ANTI-MYBPC3 (N-TERM) antibody Sigma-Aldrich Cat#SAB1303629-400UL; RRID:AB_3075444 Chemicals, peptides, and recombinant proteins Hydrogen peroxide solution Sigma Cat#88597 Phosphate Buffer Solution Sigma Cat#P3619 2-Thiobarbituric acid Sigma Cat#T5500 Glutaraldehyde solution Sigma Cat#G5882 .. Glutathione Assay Kit Cayman chemical Cat#703002 Bio-Rad Protein Assay Kit II Biorad Cat#5000002 Glucose-6-Phosphate Dehydrogenase Assay Kit (Colorimetric) Abcam Cat#ab102529 Aurum Total RNA Mini Kit Biorad Cat7326820 iScript Reverse Transcription Supermix for RT-qPCR Biorad Cat#1708841 Pierce BCA Protein Assay Kits ThermoFisher Scientific Cat# 23225 Experimental models: Organisms/strains Humanized MYBPC3 mice on C57BL/6 background This paper N/A Oligonucleotides See Table S1 for primers sequences This paper N/A Software and algorithms GraphPad Software San Diego, CA https://www.graphpad.com/ ImageJ National Institutes of Health (NIH) https://imagej.net/ij/ ..

    Article Title: Pyrazole[3,4-e][1,4]thiazepin-7-one derivatives as a novel class of Farnesoid X Receptor (FXR) agonists
    Article Snippet: A virtual screening procedure was applied to the discovery of structurally diverse non-steroidal Farnesoid X Receptor (FXR) agonists.. From 117 compounds selected by virtual screening, a total of 47 compounds were found to be FXR agonists, with 34 of them showing activity below a concentration of 20 lM.. 1H-Pyrazole[3,4-e][1,4]thiazepin-7-one-based hit compound 7 was chosen for hit-to-lead optimization.

    Quantitative RT-PCR:

    Article Title: Sleep fragmentation induces heart failure in a hypertrophic cardiomyopathy mouse model by altering redox metabolism.
    Article Snippet: Antibodies CLOCK (D45B10) Rabbit mAb Cell Signaling Technology Cat#5157; RRID:AB_10695411 BMAL1 (D2L7G) Rabbit mAb Cell Signaling Technology Cat#14020; RRID:AB_2728705 Cytochrome c (136F3) Rabbit mAb Cell Signaling Technology Cat#4280; RRID:AB_10695410 VDAC (D73D12) Rabbit mAb Cell Signaling Technology Cat#4661; RRID:AB_10557420 Cleaved Caspase-9 (Asp330) (E5Z7N) Rabbit mAb Cell Signaling Technology Cat#52873; RRID:AB_2799423 Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb Cell Signaling Technology Cat#9664; RRID:AB_2070042 Anti-NRF2 (D1Z9C) XP Rabbit monoclonal Antibody Cell Signaling Technology Cat#12721; RRID:AB_2715528 Catalase (D4P7B)XP Rabbit mAb Cell Signaling Technology Cat#12980; RRID:AB_2798079 GST-Tag (26H1) Mouse mAb Cell Signaling Technology Cat#2624; RRID:AB_2189875 GAPDH (D16H11) XP Rabbit mAb Cell Signaling Technology Cat#5174; RRID:AB_10622025 Anti-PER1 antibody - N-terminal Abcam Cat#ab136451; RRID:AB_3075441 Anti-mCherry antibody Abcam Cat# ab167453; RRID:AB_2571870 Anti-Creatine kinase MT antibody Abcam Cat#ab198257, RRID:AB_3075442 TrxR2 Recombinant Rabbit Monoclonal Antibody (ARC1339) ThermoFisher Scientific Cat#MA5-35730, RRID:AB_2849630 ANTI-MYBPC3 (N-TERM) antibody Sigma-Aldrich Cat#SAB1303629-400UL; RRID:AB_3075444 Chemicals, peptides, and recombinant proteins Hydrogen peroxide solution Sigma Cat#88597 Phosphate Buffer Solution Sigma Cat#P3619 2-Thiobarbituric acid Sigma Cat#T5500 Glutaraldehyde solution Sigma Cat#G5882 .. Glutathione Assay Kit Cayman chemical Cat#703002 Bio-Rad Protein Assay Kit II Biorad Cat#5000002 Glucose-6-Phosphate Dehydrogenase Assay Kit (Colorimetric) Abcam Cat#ab102529 Aurum Total RNA Mini Kit Biorad Cat7326820 iScript Reverse Transcription Supermix for RT-qPCR Biorad Cat#1708841 Pierce BCA Protein Assay Kits ThermoFisher Scientific Cat# 23225 Experimental models: Organisms/strains Humanized MYBPC3 mice on C57BL/6 background This paper N/A Oligonucleotides See Table S1 for primers sequences This paper N/A Software and algorithms GraphPad Software San Diego, CA https://www.graphpad.com/ ImageJ National Institutes of Health (NIH) https://imagej.net/ij/ ..

    Article Title: Drosophila Nora virus capsid proteins differ from those of other picorna-like viruses.
    Article Snippet: The recently discovered Nora virus from Drosophila melanogaster is a single-stranded RNA virus.. Its published genomic sequence encodes a typical picorna-like cassette of replicative enzymes, but no capsid proteins similar to those in other picorna-like viruses.. We have now done additional sequencing at the termini of the viral genome, extending it by 455 nucleotides at the 5′ end, but no more coding sequence was found.


    Software:

    Article Title: Sleep fragmentation induces heart failure in a hypertrophic cardiomyopathy mouse model by altering redox metabolism.
    Article Snippet: Antibodies CLOCK (D45B10) Rabbit mAb Cell Signaling Technology Cat#5157; RRID:AB_10695411 BMAL1 (D2L7G) Rabbit mAb Cell Signaling Technology Cat#14020; RRID:AB_2728705 Cytochrome c (136F3) Rabbit mAb Cell Signaling Technology Cat#4280; RRID:AB_10695410 VDAC (D73D12) Rabbit mAb Cell Signaling Technology Cat#4661; RRID:AB_10557420 Cleaved Caspase-9 (Asp330) (E5Z7N) Rabbit mAb Cell Signaling Technology Cat#52873; RRID:AB_2799423 Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb Cell Signaling Technology Cat#9664; RRID:AB_2070042 Anti-NRF2 (D1Z9C) XP Rabbit monoclonal Antibody Cell Signaling Technology Cat#12721; RRID:AB_2715528 Catalase (D4P7B)XP Rabbit mAb Cell Signaling Technology Cat#12980; RRID:AB_2798079 GST-Tag (26H1) Mouse mAb Cell Signaling Technology Cat#2624; RRID:AB_2189875 GAPDH (D16H11) XP Rabbit mAb Cell Signaling Technology Cat#5174; RRID:AB_10622025 Anti-PER1 antibody - N-terminal Abcam Cat#ab136451; RRID:AB_3075441 Anti-mCherry antibody Abcam Cat# ab167453; RRID:AB_2571870 Anti-Creatine kinase MT antibody Abcam Cat#ab198257, RRID:AB_3075442 TrxR2 Recombinant Rabbit Monoclonal Antibody (ARC1339) ThermoFisher Scientific Cat#MA5-35730, RRID:AB_2849630 ANTI-MYBPC3 (N-TERM) antibody Sigma-Aldrich Cat#SAB1303629-400UL; RRID:AB_3075444 Chemicals, peptides, and recombinant proteins Hydrogen peroxide solution Sigma Cat#88597 Phosphate Buffer Solution Sigma Cat#P3619 2-Thiobarbituric acid Sigma Cat#T5500 Glutaraldehyde solution Sigma Cat#G5882 .. Glutathione Assay Kit Cayman chemical Cat#703002 Bio-Rad Protein Assay Kit II Biorad Cat#5000002 Glucose-6-Phosphate Dehydrogenase Assay Kit (Colorimetric) Abcam Cat#ab102529 Aurum Total RNA Mini Kit Biorad Cat7326820 iScript Reverse Transcription Supermix for RT-qPCR Biorad Cat#1708841 Pierce BCA Protein Assay Kits ThermoFisher Scientific Cat# 23225 Experimental models: Organisms/strains Humanized MYBPC3 mice on C57BL/6 background This paper N/A Oligonucleotides See Table S1 for primers sequences This paper N/A Software and algorithms GraphPad Software San Diego, CA https://www.graphpad.com/ ImageJ National Institutes of Health (NIH) https://imagej.net/ij/ ..

    Isolation:

    Article Title: TGR5 modulators and methods of use thereof
    Article Snippet: .. Total RNA was isolated (Aurum Total RNA Mini Kit BioRad) from NCI-H716 stimulated with 5 μM compound 10 for 18 hours. .. The RNA was randomly reverse-transcribed with ISCRIPT cDNA SYNTHESIS KIT (BioRad) in 20 μl reaction volume.

    Article Title: Pyrazole[3,4-e][1,4]thiazepin-7-one derivatives as a novel class of Farnesoid X Receptor (FXR) agonists
    Article Snippet: A virtual screening procedure was applied to the discovery of structurally diverse non-steroidal Farnesoid X Receptor (FXR) agonists.. From 117 compounds selected by virtual screening, a total of 47 compounds were found to be FXR agonists, with 34 of them showing activity below a concentration of 20 lM.. 1H-Pyrazole[3,4-e][1,4]thiazepin-7-one-based hit compound 7 was chosen for hit-to-lead optimization.

    Concentration Assay:

    Article Title: Pyrazole[3,4-e][1,4]thiazepin-7-one derivatives as a novel class of Farnesoid X Receptor (FXR) agonists
    Article Snippet: A virtual screening procedure was applied to the discovery of structurally diverse non-steroidal Farnesoid X Receptor (FXR) agonists.. From 117 compounds selected by virtual screening, a total of 47 compounds were found to be FXR agonists, with 34 of them showing activity below a concentration of 20 lM.. 1H-Pyrazole[3,4-e][1,4]thiazepin-7-one-based hit compound 7 was chosen for hit-to-lead optimization.

    Virus:

    Article Title: Drosophila Nora virus capsid proteins differ from those of other picorna-like viruses.
    Article Snippet: The recently discovered Nora virus from Drosophila melanogaster is a single-stranded RNA virus.. Its published genomic sequence encodes a typical picorna-like cassette of replicative enzymes, but no capsid proteins similar to those in other picorna-like viruses.. We have now done additional sequencing at the termini of the viral genome, extending it by 455 nucleotides at the 5′ end, but no more coding sequence was found.

    Lysis:


    SYBR Green Assay:


    Immunoprecipitation:


    Magnetic Beads:


    Chromatin Immunoprecipitation:


    RNA Sequencing:


    Control:


    ChIP-qPCR:


    Recombinant:




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